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KMID : 0388019950060040235
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Korean Journal Gynecologic Oncology and Colposcopy
1995 Volume.6 No. 4 p.235 ~ p.243
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In Situ Hybridization of EGFR mRNA in Normal, Dysplasic, Carinoma in Situ and Malignant Cervical Tissues
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김태웅
김홍기/박종섭/김진우/강진한/이안희
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Abstract
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The specific receptor for epidermal growth factor(EGFR) was first purified from the A431 cell line which was derived from and epidermal carcinoma of the vulva. It is a 170,000 dalton transmembrane glycoprotein and has three components, an
extracellular
domain capable of binding the ligand, a transmembrane portion, and an intracellular domain facing the cytoplasm. The receptor is capable of binding epidermal growth factor(EGF), transforming growth factor(TGF) α, amphiregulin, and
heparin-binding
EGF.
The function of EGFR is to bind the mitegen EGF or TGF α and to transduce the signal across the cell membrane to the cytoplasm. The intracellular component exhibits tyrosine kinase activity and has binding sites for ATP. This results in the
autophosphorylation of the EGFR and phosphorylation of several target proteins.
It has been suggested that the EGFR is overexpressed in the A431 cell line and that there is a close correlation between EGFR, EGFR mRNA overexpression and EGFR gene amplification. However this has not been always demonstrated in cervical cancers
and
cell lines.
In this study, we compared the GEFR mRNA expression rates among normal(10 cases), dysplastic(10 cases), carcinoma in situ(CIS, 14 cases), and malignant cervical tissues(37 cases) and examed whether EGFR gene amplification is constant step in the
malignant transformation of the cervical cells. Also, we studied the relationship between the results of EGFR expression and clinicopathologic findings to confirm the usefullness of the EGFR mRNS as a prognostic factor.
@ES The results were as follows.
@EN 1. The expression rate of EGFR mRNS were 60% in normal, 70% in dysplastic, 57% in CIS, and 59% malignant cervical tissue.
2. No significant difference was seen between the clinical stages and the expression rates in malignant cervical tissue tissue group.
3. According to the cell type, the expression rates of squamous cell carcinoma was significantly higher than that adenocarcinoma(P<0.05).
4. The clinicopathologic findings were not correlated with the expression rate of the malignant cervical tissue group.
These results suggest that there is no EGFR gene amplification or EGFR overexpression on some malignant cervical tissues and that EGFR mRNA expression has less prognostic significance.
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